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citS [2017-8-11 16:0:3]
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citS [2017-8-11 16:0:3]

two-component sensor kinase, regulation of citrate uptake
locus
BSU07580
pI
6.48
mw
59.72 kDa
protein length
542 aa Sequence Blast
gene length
1626 bp Sequence Blast
function
regulation of citrate uptake
product
two-component sensor kinase
essential
no
synonyms
yflR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    830,945 → 832,573

    Phenotypes of a mutant

  • no growth with citrate as sole carbon source [Pubmed|10972810]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT]

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity may be controlled by interaction with [protein|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|YflP] [Pubmed|14499931]

    • [SW|Localization]

  • cell membrane (Heterogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]-[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]-[gene|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|yflP]
    description
    [Pubmed|10972810]
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    Biological materials

    Mutant

  • MGNA-C333 (citS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2331 NBRP B. subtilis, Japan]

    • References

    Reviews

  • 28152228

    • Original publications

  • 10094672,16842348,10972810,16479537,14499931